relationship between grade and mdm2 oncoprotein overexpression in transitional cell carcinoma of the urinary bladder

objective: transitional cell carcinoma (tcc) of the urinary bladder is the second common cancer of the genitourinary tract. several parameters such as clinical and pathological parameters, molecular factors, and etc play a role in determination of prognosis and type of treatment. in this research study, the relationship between grade and mdm2 oncoprotein overexpression in tcc of bladder was evaluated. material and methods: this cross-sectional study was carried out on 75 paraffinembedded tissue blocks (deposited in pathology department) from patients with tcc of urinary bladder from 2002 to 2005. in this respect, 3 μm sections were taken from each block. in one of the sections, grade of tumor was determined according to who/isup criteria using hematoxylin-eosin staining method. in another section, percent of mdm2 positive cells were determined by microscopic observation of 100 cells in each section using immunohistochemical technique and specific antibody. results: mean of mdm2 positive cells was %9.08 ± 4.7. in addition, mean of mdm2 positive cells in low malignant potential tissue (grade i), low grade tissue (grade ii), and high grade tissue (grade iii) was %3.6 ± 2.2, %8.2 ±3.5, and %13.1 ± 3.4 respectively. meanwhile, a significant relationship was found out between grade and overexpression of mdm2 oncoprotein (p = 0.012). conclusion: overexpression of mdm2 oncoprotein exhibits a direct relationship with grade of tcc of the urinary bladder. the obtained results were consistent with the most previous studies. therefore, through performing studies with a greater follow-up and entailing a larger population of patients, overexpression of mdm2 oncoprotein could be used as a prognostic parameter in tcc of urinary bladder.